UNLOCKING THE BOTTLENECK IN FORWARD GENETICS USING WHOLE-GENOME SEQUENCING AND IDENTITY BY DESCENT TO ISOLATE CAUSATIVE MUTATIONS.

Unlocking the bottleneck in forward genetics using whole-genome sequencing and identity by descent to isolate causative mutations.

Forward genetics screens with N-ethyl-N-nitrosourea (ENU) provide a powerful way to illuminate gene function and generate mouse models of human disease; however, the identification of causative mutations remains a limiting step.Current strategies depend on conventional mapping, so the propagation of affected mice requires non-lethal screens; accura

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Biological and chemical properties of five mineral oxides and of mineral trioxide aggregate repair high plasticity: an in vitro study

Abstract Calcium silicate-based cements have diverse applications in endodontics.This study aimed to evaluate the antibiofilm action, biocompatibility, morphological structure, chemical composition and radiopacity of Five Mineral Oxides (5MO), Mineral Trioxide Aggregate Repair High Plasticity (MTA Repair HP), and Mineral Trioxide Aggregate (MTA) ce

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